Lasers Med Sci. 2010 Jan;25(1):33-9. Epub 2009 Jan 27.
Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells.
Mvula B, Moore TJ, Abrahamse H.
Laser Research Group, Faculty of Health Science, University of Johannesburg, Doornfontein, Johannesburg, 2028, South Africa.
The study investigated the effects of low-level laser radiation and epidermal growth factor (EGF) on adult adipose-derived stem cells (ADSCs) isolated from human adipose tissue. Isolated cells were cultured to semi-confluence, and the monolayers of ADSCs were exposed to low-level laser at 5 J/cm(2) using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0 h, 24 h and 48 h after irradiation. Application of low-level laser irradiation at 5 J/cm(2) on human ADSCs cultured with EGF increased the viability and proliferation of these cells. The results indicate that low-level laser irradiation in combination with EGF enhances the proliferation and maintenance of ADSCs in vitro.
[PubMed – indexed for MEDLINE]