Stroke. 2010 Sep;41(9):2064-70. Epub 2010 Jul 8.
Biodistribution of neural stem cells after intravascular therapy for hypoxic-ischemia.
Pendharkar AV, Chua JY, Andres RH, Wang N, Gaeta X, Wang H, De A, Choi R, Chen S, Rutt BK, Gambhir SS, Guzman R.
Department of Neurosurgery, Stanford University, School of Medicine, Stanford, CA 94305-5327, USA.
Intravascular transplantation of neural stem cells represents a minimally invasive therapeutic approach for the treatment of central nervous system diseases. The cellular biodistribution after intravascular injection needs to be analyzed to determine the ideal delivery modality. We studied the biodistribution and efficiency of targeted central nervous system delivery comparing intravenous and intra-arterial (IA) administration of neural stem cells after brain ischemia.
Mouse neural stem cells were transduced with a firefly luciferase reporter gene for bioluminescence imaging (BLI). Hypoxic-ischemia was induced in adult mice and reporter neural stem cells were transplanted IA or intravenous at 24 hours after brain ischemia. In vivo BLI was used to track transplanted cells up to 2 weeks after transplantation and ex vivo BLI was used to determine single organ biodistribution.
Immediately after transplantation, BLI signal from the brain was 12 times higher in IA versus intravenous injected animals (P CONCLUSIONS:
IA transplantation results in superior delivery and sustained presence of neural stem cells in the ischemic brain in comparison to intravenous infusion.
PMID: 20616329 [PubMed – indexed for MEDLINE]